6F3S
The crystal structure of Glycogen Phosphorylase in complex with 10d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2017-10-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.763, 128.763, 116.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 116.000 - 1.900 |
R-factor | 0.13606 |
Rwork | 0.135 |
R-free | 0.15587 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.319 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.143 | |
Number of reflections | 77397 | |
<I/σ(I)> | 12.2 | 3.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.8 | 15.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer |