6F3K
Combined solid-state NMR, solution-state NMR and EM data for structure determination of the tetrahedral aminopeptidase TET2 from P. horikoshii
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR, SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE II | 600 | |
| 2 | Varian | INOVA | 800 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | RFDR 3D |
| 10 | 1 | 5 | hCANH |
| 11 | 1 | 5 | hCONH |
| 12 | 1 | 5 | hcoCAcoNH |
| 2 | 3 | 3 | DARR |
| 3 | 2 | 2 | 3D 1H-13C NOESY aliphatic |
| 4 | 3 | 4 | CCdream (CC) |
| 5 | 3 | 4 | NCO |
| 6 | 3 | 4 | NCOCX |
| 7 | 3 | 4 | NCACX |
| 8 | 3 | 4 | CONCACB |
| 9 | 3 | 4 | CANCOCX |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7.5 | 1 | 300 |
| 2 | 8 | 1 | 328 |
| 3 | 7.5 | 1 | 300 |
Conformers
| Conformers Calculated Total Number | |
| Conformers Submitted Total Number | 10 |
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.1 |
| Number of Particle | 30407 |
| Software | RELION,X-PLOR,Latitude,CTFFIND,iMODFIT |
| Symmetry | T |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
