6F3H
Crystal structure of Dss1 exoribonuclease active site mutant D477N from Candida glabrata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.370, 83.230, 110.370 |
| Unit cell angles | 106.21, 106.56, 90.93 |
Refinement procedure
| Resolution | 29.211 - 2.703 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.24030 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.211 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.173 | 0.938 |
| Number of reflections | 129398 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M MES monohydrate (pH 6.5) and 6% (w/v) PEG 20000 |
