6F39
C1r homodimer CUB1-EGF-CUB2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.563, 54.620, 138.180 |
Unit cell angles | 90.00, 99.90, 90.00 |
Refinement procedure
Resolution | 68.061 - 5.801 |
R-factor | 0.3096 |
Rwork | 0.308 |
R-free | 0.33790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.357 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2722) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.100 | 6.000 |
High resolution limit [Å] | 5.800 | 5.800 |
Rpim | 0.118 | |
Number of reflections | 2987 | |
<I/σ(I)> | 5.1 | 1.8 |
Completeness [%] | 99.8 | |
Redundancy | 15.2 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 12-18% PEG 8000, 100 mM Imidazole at pH 8.0 |