6F2R
A heterotetramer of human HspB2 and HspB3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 |
| Unit cell lengths | 177.139, 177.139, 126.461 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.000 - 3.900 |
| R-factor | 0.298 |
| Rwork | 0.297 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ENSEMBLE OF 3Q9P 2wj5 2wj7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.460 | 4.110 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.131 | 1.163 |
| Number of reflections | 147958 | |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 294 | 0.6M SODIUM FORMATE, 0.1 M TRIS HCL pH7.8, 7.8% PGA, 2mM GTP |
