6F2J

Crystal structure of Hen Egg-White Lysozyme co-crystallized in presence of 100 mM Tb-Xo4 and 100 mM sodium sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9797
Spacegroup name	P 43 21 2
Unit cell lengths	77.915, 77.915, 37.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.840 - 1.300
R-factor	0.16
Rwork	0.159
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h87
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.960	1.370
High resolution limit [Å]	1.300	1.300
Rpim	0.023	0.253
Number of reflections	29089
<I/σ(I)>	22.3
Completeness [%]	99.8
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Sodium acetate pH 4.6, 200mM to 800 mM sodium chloride and 100 mM sodium sulfate. Tb-Xo4 was directly mixed with the protein solution at a final concentration of 100 mM prior to crystallization.