6F2I

Crystal structure of Hen Egg-White Lysozyme co-crystallized in presence of 100 mM Tb-Xo4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97973
Spacegroup name	P 43 21 2
Unit cell lengths	78.149, 78.149, 37.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.070 - 1.200
R-factor	0.174
Rwork	0.173
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h87
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.080
High resolution limit [Å]	1.200
Rpim	0.029
Number of reflections	37176
<I/σ(I)>	12.4
Completeness [%]	99.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Sodium acetate pH 4.6, 0.2 to 0.8 M Chlorure de Sodium. Tb-Xo4 was directly mixed with the protein solution at a final concentration of 100 mM prior to crystallization.