6F2G

Bacterial asc transporter crystal structure in open to in conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-10-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979260
Spacegroup name	P 41 21 2
Unit cell lengths	85.830, 85.830, 321.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.050 - 2.920
R-factor	0.2395
Rwork	0.238
R-free	0.26123
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dji 4krn
RMSD bond length	0.017
RMSD bond angle	1.965
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.050	3.080
High resolution limit [Å]	2.920	2.920
Rmerge	0.058	0.885
Rmeas	0.065	1.117
Rpim	0.028	0.673
Number of reflections	26615	3650
<I/σ(I)>	12.6	1.3
Completeness [%]	98.2	95
Redundancy	5.5	4.6
CC(1/2)	0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	28%PEG400 0,1M 0,1M Ammonium acetate pH8