6F1R
Tetragonal Lysozyme crystallized at 298 K and pH 4.5 with phosphate bound: control experiment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.694, 78.694, 37.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.438 - 1.100 |
R-factor | 0.1542 |
Rwork | 0.154 |
R-free | 0.16350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e3u |
RMSD bond length | 0.006 |
RMSD bond angle | 0.928 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.830 | 18.830 | 1.120 |
High resolution limit [Å] | 1.100 | 6.020 | 1.100 |
Rmerge | 0.047 | 0.040 | 0.603 |
Rmeas | 0.050 | 0.043 | 0.644 |
Rpim | 0.018 | 0.017 | 0.223 |
Total number of observations | 396478 | ||
Number of reflections | 48271 | 267 | 2378 |
<I/σ(I)> | 20.2 | ||
Completeness [%] | 99.6 | 72.8 | 100 |
Redundancy | 8.2 | 6.5 | 8 |
CC(1/2) | 0.998 | 0.995 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.5 M Sodium Chloride, 0.1 M Sodium Acetate |