6F1P

Tetragonal Lysozyme crystallized at 298 K and pH 4.5 with phosphate bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 43 21 2
Unit cell lengths	77.056, 77.056, 38.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.689 - 1.120
R-factor	0.1543
Rwork	0.154
R-free	0.16770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e3u
RMSD bond length	0.006
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.190	19.190	1.140
High resolution limit [Å]	1.120	6.130	1.120
Rmerge	0.070	0.069	0.628
Rmeas	0.073	0.073	0.688
Rpim	0.022	0.024	0.272
Number of reflections	44928	320	2135
<I/σ(I)>	15.9
Completeness [%]	99.8	95	97.9
Redundancy	10.5	8.8	6
CC(1/2)	0.998	0.995	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.2 M Sodium Chloride, 0.1 M Sodium Acetate