6F1O
Orthorhombic Lysozyme crystallized at 298 K and pH 4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.062, 56.176, 73.289 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.963 - 0.960 |
R-factor | 0.1317 |
Rwork | 0.131 |
R-free | 0.14160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e3u |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.730 | 18.730 | 0.980 |
High resolution limit [Å] | 0.960 | 5.260 | 0.960 |
Rmerge | 0.050 | 0.051 | 0.791 |
Rmeas | 0.053 | 0.055 | 0.848 |
Rpim | 0.017 | 0.018 | 0.295 |
Total number of observations | 763574 | ||
Number of reflections | 76153 | 502 | 3307 |
<I/σ(I)> | 23.1 | ||
Completeness [%] | 99.3 | 92.7 | 88.4 |
Redundancy | 10 | 9.2 | 7.5 |
CC(1/2) | 0.998 | 0.993 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.2 M Sodium Chloride, 0.1 M Sodium Acetate |