6F1M
Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5: low-humidity form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.104, 55.522, 66.007 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.497 - 1.480 |
R-factor | 0.2275 |
Rwork | 0.226 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e3u |
RMSD bond length | 0.008 |
RMSD bond angle | 0.992 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.500 | 19.500 | 1.500 |
High resolution limit [Å] | 1.480 | 8.090 | 1.480 |
Rmerge | 0.092 | 0.055 | 0.527 |
Rmeas | 0.102 | 0.061 | 0.595 |
Rpim | 0.043 | 0.026 | 0.266 |
Number of reflections | 17334 | 117 | 933 |
<I/σ(I)> | 9 | ||
Completeness [%] | 90.9 | 83 | 98.3 |
Redundancy | 4.8 | 4.8 | 4.6 |
CC(1/2) | 0.994 | 0.993 | 0.664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.5 M Sodium Chloride, 0.1 M Sodium Phosphate. 0.1 M Sodium Acetate |