6F1J
Structure of a Talaromyces pinophilus GH62 Arabinofuranosidase in complex with AraDNJ at 1.25A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.827, 88.978, 72.663 |
| Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
| Resolution | 33.520 - 1.250 |
| R-factor | 0.12055 |
| Rwork | 0.120 |
| R-free | 0.13588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.499 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.520 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rpim | 0.040 | 0.280 |
| Number of reflections | 149344 | 6813 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 98.0 | 91 |
| Redundancy | 3.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG2K MME, 0.2M KBr |
