6F1G
The structure of AbnB-E201A, an intracellular 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus, in complex with arabinopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.960, 89.210, 40.839 |
| Unit cell angles | 90.00, 102.45, 90.00 |
Refinement procedure
| Resolution | 29.730 - 1.672 |
| R-factor | 0.1622 |
| Rwork | 0.160 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d5y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.079 | 0.530 |
| Number of reflections | 34562 | 1699 |
| <I/σ(I)> | 28.8 | 1.9 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.9-2.1 M LiSO4, 0.1 M Tris pH 8.5, 3% PEG400, 5mM arabinopentose |
