6F1E

Crystal structure of olive flounder [Paralichthys olivaceus] interferon gamma at 2.3 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	58.238, 79.721, 94.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.590 - 2.296
R-factor	0.22898
Rwork	0.226
R-free	0.29666
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.599
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.600	47.300	2.380
High resolution limit [Å]	2.296	8.590	2.296
Rmerge	0.102	0.037	2.711
Rmeas	0.110	0.040	2.956
Rpim	0.042	0.016	1.163
Number of reflections	20255	450	2032
<I/σ(I)>	13.5	48.1	0.9
Completeness [%]	99.6	98.5	97.4
Redundancy	12.9	9.9	11.9
CC(1/2)	0.999	0.999	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Protein buffer: 100 mM citrate buffer, pH 6.0 Reservoir: 3 M NaCl 0.1 M Bis-Tris, pH 5.5