6F16
GLIC mutant H277Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2016-05-04 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.700, 132.780, 161.550 |
| Unit cell angles | 90.00, 102.96, 90.00 |
Refinement procedure
| Resolution | 21.300 - 2.600 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.21920 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 115236 | |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 100 mM NaAcetate pH 4 400 NaSCN 12-15% PEG4000 15% glycerol 3%DMSO |
