6F16
GLIC mutant H277Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2016-05-04 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 182.700, 132.780, 161.550 |
Unit cell angles | 90.00, 102.96, 90.00 |
Refinement procedure
Resolution | 21.300 - 2.600 |
R-factor | 0.2022 |
Rwork | 0.201 |
R-free | 0.21920 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.480 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 115236 | |
<I/σ(I)> | 12.7 | |
Completeness [%] | 99.9 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 100 mM NaAcetate pH 4 400 NaSCN 12-15% PEG4000 15% glycerol 3%DMSO |