6F13
GLIC mutant E75A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2015-04-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.948, 132.450, 159.234 |
Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.700 |
R-factor | 0.2037 |
Rwork | 0.203 |
R-free | 0.22270 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 100065 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.7 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 100 mM NaAcetate pH 4 200mM NaSCN 12-15% PEG4000 15% glycerol 3%DMSO |