6F12
GLIC mutant E181A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2015-11-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.210, 132.140, 159.920 |
Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.2014 |
Rwork | 0.201 |
R-free | 0.21340 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.630 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 61659 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 99.9 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 100 mM NaAcetate pH4 400 NaSCN 12-15% PEG4000 15% glycerol 3%DMSO |