6F11
GLIC mutant D86A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2015-10-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9780 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.820, 133.790, 160.160 |
| Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.1961 |
| Rwork | 0.195 |
| R-free | 0.21550 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.440 | 3.010 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 77611 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 100 mM NaAcetate pH4 400 mM NaSCN 12-15% PEG 4000 15% glycerol 3%DMSO |
