6F10
GLIC mutant D88N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2015-09-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 183.370, 134.810, 160.680 |
Unit cell angles | 90.00, 103.12, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.850 |
R-factor | 0.2082 |
Rwork | 0.207 |
R-free | 0.22270 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 87226 | |
<I/σ(I)> | 26.4 | |
Completeness [%] | 99.9 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 100 mM NaAcetate pH4 400 mM NaSCN 12-15% PEG4000 15% glycerol 3%DMSO |