6F0U
GLIC mutant E35A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2015-02-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.840, 133.060, 158.390 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 27.140 - 2.350 |
| R-factor | 0.2183 |
| Rwork | 0.217 |
| R-free | 0.23260 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 148853 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 97.6 | |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO |
