6F0R
GLIC mutant E82Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2015-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.840, 132.420, 160.060 |
| Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.25400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 112433 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO |
