6F0Q
Crystal structure of Pizza6-AYW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.010, 70.350, 58.210 |
| Unit cell angles | 90.00, 100.60, 90.00 |
Refinement procedure
| Resolution | 26.501 - 1.300 |
| R-factor | 0.1571 |
| Rwork | 0.155 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ww9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.501 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.062 | 0.301 |
| Rpim | 0.062 | 0.301 |
| Number of reflections | 86627 | |
| <I/σ(I)> | 8.2 | 2.8 |
| Completeness [%] | 94.7 | 92.6 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M MIB (Mixtures of sodium malonate, imidazole, boric acid in the molar rations 2:3:3 buffer) or MMT (Mixtures of DL-malic acid, MES and Tris base in the molar rations 1:2:2 buffer) pH 5.0, 30% (w/v) PEG1500 |
