6F0I
GLIC mutant E26A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2015-05-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.067, 133.412, 159.241 |
| Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| R-factor | 0.2087 |
| Rwork | 0.208 |
| R-free | 0.22260 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 74072 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 291.15 | PEG 4000, 15% Glycerol, 0.1M Sodium acetate, 0.4M Thiocyanate, 3% DMSO |
