6F0H
Crystal structure ASF1-ip4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 73.440, 73.440, 229.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.860 - 1.980 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2io5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP (11.0.02) |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 1.750 | 1.980 |
| Rmerge | 0.016 | 0.056 |
| Number of reflections | 26500 | |
| <I/σ(I)> | 14.3 | 6.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.9 | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Citrate 0.1M pH 2.5, LiSO4 0.5M, PEG8000 10% |
