6F0E

Structure of yeast Sec14p with a picolinamide compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2017-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	88.077, 88.077, 109.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.412 - 2.600
R-factor	0.2006
Rwork	0.199
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aua
RMSD bond length	0.017
RMSD bond angle	1.369
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.600	2.600
Rmeas	0.227
Number of reflections	29163
<I/σ(I)>	12.22
Completeness [%]	99.9
Redundancy	10.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	129.5 mM sodium acetate, 64.8 mM TRIS, 10 % (w/v) PEG 4000, 20 % (v/v) glycerol, pH 7.0