6F03
The crystal structure of secreted antigen BPSL2520
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.955, 52.731, 136.122 |
| Unit cell angles | 90.00, 93.84, 90.00 |
Refinement procedure
| Resolution | 38.954 - 2.200 |
| R-factor | 0.2045 |
| Rwork | 0.202 |
| R-free | 0.24450 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.861 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((1.10.1_2155-0000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.122 | 0.636 |
| Number of reflections | 36157 | 35764 |
| <I/σ(I)> | 8.9 | 3.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30 % PEG 4000, 0.2 M ammonium acetate, 0.1M HEPES pH 7.0. 20% glycerol added for cryoprotection |
