6F01

ARABIDOPSIS THALIANA GSTF9, GSO3 AND GSOH BOUND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	114.760, 114.760, 90.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.837 - 2.500
R-factor	0.1666
Rwork	0.164
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GSTF9
RMSD bond length	0.010
RMSD bond angle	1.268
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.840	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.177	1.218
Number of reflections	24179
<I/σ(I)>	11.56	2.09
Completeness [%]	99.8	99.9
Redundancy	6.5	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	0.1M KBr 30% PEGMME 2000