6EZY

ARABIDOPSIS THALIANA GSTF9, GSH AND GSOH BOUND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	114.125, 114.125, 89.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.418 - 2.350
R-factor	0.1583
Rwork	0.156
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ri6
RMSD bond length	0.012
RMSD bond angle	1.267
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.418	2.480
High resolution limit [Å]	2.340	2.340
Rmerge		0.112
Number of reflections	28880
<I/σ(I)>	18.3	1.64
Completeness [%]	98.7	96.8
Redundancy	9.9	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	0.10M KBr, 30% PEGMME 2000