6EZW
Crystal structure of a llama VHH antibody BCD090-M2 against human ErbB3 in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-12 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.759, 38.931, 47.482 |
| Unit cell angles | 90.00, 102.24, 90.00 |
Refinement procedure
| Resolution | 33.298 - 1.598 |
| R-factor | 0.185278623977 |
| Rwork | 0.182 |
| R-free | 0.21256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5imk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.823 |
| Data reduction software | SAINT (V8.18C) |
| Data scaling software | SADABS (2008/1) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.298 | 33.305 | 1.626 |
| High resolution limit [Å] | 1.598 | 4.336 | 1.598 |
| Rmerge | 0.073 | 0.044 | 0.387 |
| Rmeas | 0.081 | ||
| Rpim | 0.033 | ||
| Number of reflections | 15511 | 818 | 592 |
| <I/σ(I)> | 12.7359219637 | 47.9552706203 | 2.0696012381 |
| Completeness [%] | 98.8 | ||
| Redundancy | 5.21442847012 | 10.2995110024 | 2.29898648649 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | Reservoir solution: 0.1M MES-Imidazole pH 6.5, 0.02M Sodium formate, 0.02M Ammonium acetate, 0.02M Sodium citrate, 0.02M Sodium potassium tartrate, 0.02M Sodium oxamate, 20% v/v PEG 500MME, 10% w/v PEG 20 000 |
