6EZS
Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97889 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.250, 129.630, 100.000 |
| Unit cell angles | 90.00, 114.36, 90.00 |
Refinement procedure
| Resolution | 48.020 - 2.500 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ezr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.020 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 72950 | 5403 |
| <I/σ(I)> | 9.71 | 3.17 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.7 | 4.53 |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 293 | 0.1 M sodium acetate pH 4.6, 1.4 M sodium malonate |
