6EZR
Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9998 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.150, 130.710, 98.480 |
Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
Resolution | 90.660 - 2.370 |
R-factor | 0.2166 |
Rwork | 0.214 |
R-free | 0.25910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rcn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.272 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.660 | 90.660 | 2.430 |
High resolution limit [Å] | 2.370 | 10.600 | 2.370 |
Rmerge | 0.146 | 0.058 | 1.087 |
Rmeas | 0.159 | 0.063 | 1.173 |
Total number of observations | 577490 | ||
Number of reflections | 84783 | 985 | 6189 |
<I/σ(I)> | 9.93 | 21.25 | 2.21 |
Completeness [%] | 99.4 | 97.8 | 99.1 |
Redundancy | 6.811 | 6.621 | 7.051 |
CC(1/2) | 0.994 | 0.997 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.5 | 293 | 0.1 M sodium acetate pH 4.6, 1.4 M sodium malonate |