6EZ3
Structure of CDPS from Staphylococcus haemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.381, 81.835, 384.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 192.010 - 3.000 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 192.013 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.913 | |
| Number of reflections | 30525 | |
| <I/σ(I)> | 9.91 | 1.89 |
| Completeness [%] | 99.4 | 98.1 |
| Redundancy | 6.09 | 6.36 |
| CC(1/2) | 0.997 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG4000 0.2 M LiSO4 0.1M Tris pH 8.5 |
