6EYV
Crystal structure of the pyoverdine maturation protein PvdP in complex with the mock substrates L-tyrosine and zinc.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-13 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.282 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.332, 109.143, 82.510 |
Unit cell angles | 90.00, 95.66, 90.00 |
Refinement procedure
Resolution | 48.092 - 2.704 |
R-factor | 0.2096 |
Rwork | 0.205 |
R-free | 0.26180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6eys |
RMSD bond length | 0.002 |
RMSD bond angle | 0.460 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2875)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.092 | 3.032 |
High resolution limit [Å] | 2.701 | 2.701 |
Rmeas | 0.320 | |
Rpim | 0.101 | 0.536 |
Number of reflections | 23084 | 1154 |
<I/σ(I)> | 8.6 | 1.6 |
Completeness [%] | 92.2 | 56.5 |
Redundancy | 9.9 | 7.5 |
CC(1/2) | 0.988 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.857 M NH4SO4 0.1 M MES pH 5.5 0.5 M ZnCl2 1 mM L-tyrosine |