6EYS
Crystal structure of the periplasmic pyoverdine maturation protein PvdP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.353, 107.793, 107.945 |
Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
Resolution | 45.606 - 2.091 |
R-factor | 0.2147 |
Rwork | 0.214 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.495 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2875)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.072 | 2.259 |
High resolution limit [Å] | 2.091 | 2.091 |
Rpim | 0.051 | 0.499 |
Number of reflections | 85942 | 4285 |
<I/σ(I)> | 10.7 | 1.6 |
Completeness [%] | 93.2 | 76.7 |
Redundancy | 6.7 | 6.5 |
CC(1/2) | 0.997 | 0.537 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20%PEG3350 0.18M tri-Ammoniumcitrat |