6EYS
Crystal structure of the periplasmic pyoverdine maturation protein PvdP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.353, 107.793, 107.945 |
| Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
| Resolution | 45.606 - 2.091 |
| R-factor | 0.2147 |
| Rwork | 0.214 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.495 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2875)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.072 | 2.259 |
| High resolution limit [Å] | 2.091 | 2.091 |
| Rpim | 0.051 | 0.499 |
| Number of reflections | 85942 | 4285 |
| <I/σ(I)> | 10.7 | 1.6 |
| Completeness [%] | 93.2 | 76.7 |
| Redundancy | 6.7 | 6.5 |
| CC(1/2) | 0.997 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20%PEG3350 0.18M tri-Ammoniumcitrat |
