6EYS

Crystal structure of the periplasmic pyoverdine maturation protein PvdP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	97.353, 107.793, 107.945
Unit cell angles	90.00, 99.97, 90.00

Refinement procedure

Resolution	45.606 - 2.091
R-factor	0.2147
Rwork	0.214
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.495
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((dev_2875))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.072	2.259
High resolution limit [Å]	2.091	2.091
Rpim	0.051	0.499
Number of reflections	85942	4285
<I/σ(I)>	10.7	1.6
Completeness [%]	93.2	76.7
Redundancy	6.7	6.5
CC(1/2)	0.997	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20%PEG3350 0.18M tri-Ammoniumcitrat