6EYL
Crystal structure of OpuBC in complex with carnitine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-17 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.8726 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.710, 66.610, 126.990 |
Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
Resolution | 42.300 - 1.500 |
R-factor | 0.1629 |
Rwork | 0.161 |
R-free | 0.19240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r6u |
RMSD bond length | 0.006 |
RMSD bond angle | 1.075 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 79124 | 7903 |
<I/σ(I)> | 9.2 | 4.1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 5 | 273 | PEG 3350 phosphate buffer |