6EYG
Structure of a OpuBC mutant with bound Glycine betaine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-17 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.8726 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.750, 66.210, 63.590 |
Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
Resolution | 63.400 - 1.420 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.21933 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r6u |
RMSD bond length | 0.022 |
RMSD bond angle | 2.145 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.400 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 45559 | 4452 |
<I/σ(I)> | 10.2 | |
Completeness [%] | 98.2 | 97.3 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 5 | 273 | 150 to 225 mM potassium acetate and 20% to 27.5% polyethylene glycol 3350. |