6EXR

CHEMOTAXIS PROTEIN CHEY FROM Pyrococcus horikoshiI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9334
Spacegroup name	C 1 2 1
Unit cell lengths	109.140, 124.370, 73.420
Unit cell angles	90.00, 111.75, 90.00

Refinement procedure

Resolution	68.193 - 2.160
Rwork	0.242
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u0s
RMSD bond length	0.016
RMSD bond angle	1.792
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158 2016/10/03)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.190	2.200
High resolution limit [Å]	2.160	2.160
Rmerge	0.050	1.500
Rmeas	0.062	1.600
Rpim	0.023	0.600
Number of reflections	47836	1953
<I/σ(I)>	22.8	1.2
Completeness [%]	98.2	82
Redundancy	7.4	5.7
CC(1/2)	1.000	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M Tris-HCl, 1.2 M sodium malonate