6EXP

Crystal structure of the SIRV3 AcrID1 (gp02) anti-CRISPR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98020
Spacegroup name	P 1 21 1
Unit cell lengths	54.750, 80.440, 106.320
Unit cell angles	90.00, 101.23, 90.00

Refinement procedure

Resolution	26.890 - 1.930
R-factor	0.236476965072
Rwork	0.235
R-free	0.26902
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.206
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.890	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.036	0.978
Rmeas	0.043	1.170
Number of reflections	67149	3320
<I/σ(I)>	13.2	1.2
Completeness [%]	98.7	97.8
Redundancy	3.4	3.2
CC(1/2)	0.999	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	18-20% (w/v) PEG 8000, 0.2 M MgCl2, 0.1 M Tris-Cl, pH 8.5