6EXD
Crystal structure of DotM cytoplasmic domain (residues 153-380) SeMet derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.000, 118.000, 66.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.140 |
| R-factor | 0.19303 |
| Rwork | 0.191 |
| R-free | 0.22749 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.084 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BUCCANEER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.160 |
| Number of reflections | 29190 | |
| <I/σ(I)> | 9.11 | 1.5 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 13.2 | 12.6 |
| CC(1/2) | 0.970 | 0.415 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M MES pH 6.0 0.15 M NH4SO4 25 % w/v PEG 4000 |
