6EXC
Crystal structure of DotM cytoplasmic domain (residues 153-380),R314E/R315E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 46.430, 50.630, 55.440 |
Unit cell angles | 102.52, 97.67, 95.50 |
Refinement procedure
Resolution | 53.440 - 2.160 |
R-factor | 0.1995 |
Rwork | 0.196 |
R-free | 0.26013 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6exb |
RMSD bond length | 0.014 |
RMSD bond angle | 1.735 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.440 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 25233 | |
<I/σ(I)> | 5.7 | 1.3 |
Completeness [%] | 97.1 | 95.3 |
Redundancy | 1.8 | 1.7 |
CC(1/2) | 0.494 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M potassium chloride 0.1 M HEPES pH 7.5 15% w/v PEG 6000 |