6EXB
Crystal structure of DotM cytoplasmic domain (residues 153-380), native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.520, 118.520, 66.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 102.700 - 1.840 |
| R-factor | 0.20405 |
| Rwork | 0.202 |
| R-free | 0.25034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6exd |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.944 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.700 | 1.890 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 46854 | |
| <I/σ(I)> | 13.9 | 1.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.1 | 9.3 |
| CC(1/2) | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium citrate pH 5.0 a 15% w/v PEG 6000 |
