6EXA
Crystal structure of DotM cytoplasmic domain (residues 153-380), double mutant R196E/R197E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.466, 118.466, 66.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.470 - 1.790 |
| R-factor | 0.20508 |
| Rwork | 0.203 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6exb |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.955 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.470 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 50188 | |
| <I/σ(I)> | 12 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M lithium sulphate 0.1 M MES pH 6.0 20% w/v PEG 4000 |
