6EX6
The GH127, Beta-arabinofuranosidase, BT3674
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 136.821, 136.821, 135.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.750 - 2.160 |
| R-factor | 0.17844 |
| Rwork | 0.176 |
| R-free | 0.22072 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wkx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.912 |
| Data reduction software | xia2 |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.400 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Number of reflections | 77030 | 5696 |
| <I/σ(I)> | 12.9 | 1.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11.5 | 11.6 |
| CC(1/2) | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris buffer, 0.2M LiSO4, 40% PEG400 |
