6EWY

RipA Peptidoglycan hydrolase (Rv1477, Mycobacterium tuberculosis) N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	C 2 2 2
Unit cell lengths	44.916, 93.960, 138.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.987 - 2.200
R-factor	0.2303
Rwork	0.228
R-free	0.26670
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.913
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.987	2.270
High resolution limit [Å]	2.191	2.191
Rmerge	0.056	0.381
Rmeas	0.062	0.420
Rpim	0.026	0.174
Number of reflections	12284	614
<I/σ(I)>	14.5	4.1
Completeness [%]	90.5	87.3
Redundancy	5.8	5.3
CC(1/2)	0.995	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.75	277	42% MPD, 0.1M MES pH 5.75