6EWQ
Putative sugar aminotransferase Spr1654 from Streptococcus pneumoniae, PLP-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.008, 99.324, 107.608 |
| Unit cell angles | 90.00, 97.74, 90.00 |
Refinement procedure
| Resolution | 29.700 - 2.200 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.614 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.098 | 0.501 |
| Number of reflections | 79389 | 7743 |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 95.0 | |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M Sodium acetate trihydrate 0.1 M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8 000 |
