6EWP

Mus musculus CEP120 third C2 domain (C2C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 61
Unit cell lengths	107.008, 107.008, 141.762
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.300 - 1.850
R-factor	0.20754
Rwork	0.207
R-free	0.22653
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.319
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	REFMAC (5.8.0174)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.300	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.081
Rmeas	0.088	0.995
Rpim	0.034	0.390
Number of reflections	78276	4455
<I/σ(I)>	11.8	1.9
Completeness [%]	100.0	100
Redundancy	6.4	6.4
CC(1/2)	0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	292	50 mM Na-MES, pH 5.2 10 mM MgCl2 200 mM KCl 5 % (w/v) PEG-8000