6EWN
HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | I 41 |
| Unit cell lengths | 88.650, 88.650, 114.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.030 - 2.290 |
| Rwork | 0.280 |
| R-free | 0.34190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gme |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.030 | 2.410 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.093 | 0.247 |
| Rmeas | 0.107 | 0.278 |
| Rpim | 0.051 | 0.124 |
| Number of reflections | 19883 | 2887 |
| <I/σ(I)> | 18.2 | 15.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4.8 |
| CC(1/2) | 0.992 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10mg/ml protein, 30%PEG MME, Tris-HCl pH=8.0 |
