6EWN
HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | I 41 |
Unit cell lengths | 88.650, 88.650, 114.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.030 - 2.290 |
Rwork | 0.280 |
R-free | 0.34190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gme |
Data reduction software | SCALA |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.030 | 2.410 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.093 | 0.247 |
Rmeas | 0.107 | 0.278 |
Rpim | 0.051 | 0.124 |
Number of reflections | 19883 | 2887 |
<I/σ(I)> | 18.2 | 15.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4 | 4.8 |
CC(1/2) | 0.992 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10mg/ml protein, 30%PEG MME, Tris-HCl pH=8.0 |