6EWH

Oreochromis niloticus CEP120 second C2 domain (C2B) G307S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	36.449, 67.250, 89.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.170 - 1.500
R-factor	0.185
Rwork	0.184
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.829
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.470	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.037
Number of reflections	36042
<I/σ(I)>	17.2	1.8
Completeness [%]	100.0	100
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	292	100 mM Na-MES, pH 6.1 29 % (v/v) PEG-400