6EWC
Crystal structure of non-phosphorylated form of RLS PHOSPHOPEPTIDE BOUND TO HLA-A2 in complex with LILRB1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-09 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5417 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 117.500, 117.500, 203.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.580 - 3.200 |
R-factor | 0.2076 |
Rwork | 0.206 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p7q |
RMSD bond length | 0.008 |
RMSD bond angle | 1.195 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.580 | 19.580 | 3.300 |
High resolution limit [Å] | 3.200 | 10.000 | 3.200 |
Rmerge | 0.173 | 0.047 | 0.442 |
Rmeas | 0.191 | 0.052 | 0.489 |
Total number of observations | 149740 | ||
Number of reflections | 26415 | 743 | 2338 |
<I/σ(I)> | 10.73 | 29.36 | 3.89 |
Completeness [%] | 96.0 | 73.3 | 98.6 |
Redundancy | 5.669 | 5.829 | 5.548 |
CC(1/2) | 0.979 | 0.998 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 12.8% PEG 3350, 3% Tacsimate, 10% dimethyl sulphoxide |